Generator

Part:BBa_K4462111:Design

Designed by: Kredai Raaman   Group: iGEM22_IISc-Bengaluru   (2022-09-30)


pTOD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 450
    Illegal NgoMIV site found at 648
    Illegal NgoMIV site found at 1498
    Illegal NgoMIV site found at 3256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3622


Design Notes

Source

Assembly

References

BRENDA Enzyme Database (brenda-enzymes.org) Alagappan, G., & Cowan, R. M. (2004). Effect of temperature and dissolved oxygen on the growth kinetics of pseudomonas putida F1 growing on benzene and toluene. Chemosphere, 54(8), 1255–1265. https://doi.org/10.1016/j.chemosphere.2003.09.013 Zylstra, G. J., Wackett, L. P., & Gibson, D. T. (1989). Trichloroethylene degradation by escherichia coli containing the cloned pseudomonas putida F1 toluene dioxygenase genes. Applied and Environmental Microbiology, 55(12), 3162–3166. https://doi.org/10.1128/aem.55.12.3162-3166.1989 Zylstra GJ, Gibson DT. Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. J Biol Chem. 1989 Sep 5;264(25):14940-6. PMID: 2670929.